Results yielded surprising evidence of interactions between the CB2 receptor selective agonist O-1966 and morphine that were dependent upon the order of administration. The effects of O-1966 on mu-opioid receptor binding were determined using DAMGO and GTPγS binding assays, and these interactions were further examined by FRET analysis linked to flow cytometry. Co-administration of morphine and O-1966 was tested under three dosing regimens: simultaneous administration, morphine pre-treated with O-1966, and O-1966 pre-treated with morphine. In the present set of experiments, we tested both the acute and chronic interactions between morphine and the CB2 receptor selective agonist O-1966 treatments on antinociception and antinociceptive tolerance in C57Bl6 mice. CB2 receptor activation has also been shown to reduce morphine-induced hyperalgesia in rodents, an effect attributed to CB2 receptor modulation of inflammation. Activation of cannabinoid CB2 receptors reverses allodynia and hyperalgesia in models of chronic pain, and co-administration of morphine with CB2 receptor selective agonists has been shown to be synergistic. 3Center for Inflammation, Translational, and Clinical Lung Research, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, United StatesĪcutely, non-selective cannabinoid (CB) agonists have been shown to increase morphine antinociceptive effects, and we and others have also demonstrated that non-selective CB agonists attenuate morphine antinociceptive tolerance.2Department of Gastroenterology and Hepatology, Temple University Hospital, Philadelphia, PA, United States. 1Center for Substance Abuse Research (CSAR), Department of Neural Sciences, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, United States.Tallarida 1 Thomas Rogers 3 Lee-Yuan Liu-Chen 1 Ronald F. Reichenbach 1,2 Kelly DiMattio 1 Suren Rajakaruna 3 David Ambrose 3 William D.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |